Srdx as a suppressor for transcription factors

Д¬hung K, Sakamoto S, Mitsuda N et al (2016b) WUSCHEL-RELATED HOMEOBOX 2 is a transcriptional repressor involved in lateral organ formation and separation in Arabidopsis. Д¬hung K, Nakano T, Fujiwara S et al (2016a) The ERF transcription factor EPI1 is a negative regulator of dark-induced and jasmonate-stimulated senescence in Arabidopsis. Д¬hen Z, Liu Y, Yin Y et al (2019) Expression of AtGA2ox1 enhances drought tolerance in maize. Д¬hen JH, Jiang HW, Hsieh EJ et al (2012) Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid. Д«arker R, Fernandez Garcia MN, Powers SJ et al (2021) Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip. Д«ai MY, Fan M, Oh E et al (2012) A triple helix-loop-helix/basic helix-loop-helix cascade controls cell elongation downstream of multiple hormonal and environmental signaling pathways in Arabidopsis. Altogether, our findings provide new insights into the possible functions of AtERF4 in Arabidopsis growth and development, and in mediating plant responses to abiotic stresses.ДЄrsovski AA, Haughn GW, Western TL (2010) Seed coat mucilage cells of Arabidopsis thaliana as a model for plant cell wall research. Expression of AtERF4-SRDX also affected abiotic stress tolerance of transgenic plants, inducing enhanced resistance to drought but reduced tolerance to salinity and heat, which could be linked to abscisic acid signaling. The short primary root and the elongation of petioles and stems were due to reduced and enhanced lengthwise cell expansion respectively, while the redistribution of seed mucilage appeared to be the result of altered pectin methylesterase activity. Here, we demonstrate that transgenic Arabidopsis containing a chimeric AtERF4-SRDX construct display a variety of changes in plant growth and development, such as short primary roots and root hairs at early developmental stages, an increased number of rosette leaves with decreased leaf area, elongation of leaf petioles and inflorescence stems, prolonged growth period, reduced stem and leaf angle and a modified seed coat mucilage structure. Transcription factors (TFs) fused to the SRDX (a modified repressor domain at the C-terminal region of Arabidopsis SUPERMAN (SUPRD) with the sequence of LDLDLELRLGFA) motif repression domain at their C terminus, generating a chimeric repressor, could act as a dominant suppressor and overcome problems related to functional redundancy between TFs.

Srdx as a suppressor for transcription factors